Z-PETH reacts primarily with phosphatidylethanol (of synthetic or natural origin), and demonstrates low cross-reactivity with other lipids and phospholipids including phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, phosphatidylserine, phosphatidylglycerol, sphingomyelin, and cholesterol. The following applications have been demonstrated by Echelon Biosciences: ELISA (0.5 μg/mL) and fluorescent microscopy (2.5 μg/mL). Z-PETH recognizes phosphatidylethanol (PEth), biotinylated PEth, or fluorescent PEth depending on the application. Additional in vitro and cellular applications are possible using this antibody, but have not been verified by Echelon. |