产品介绍
| Full-length recombinant human PI4KIIB was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Enzyme obtained from SignalChem. Molecular weight: ~84 kDa Sequence: Full length Species: Human Tag: GST tag Purity: >90 % (see TDS for lot specific information) Specific Activity: >30 nmol/min/mg (see TDS for lot specific information) Expression System: Sf9 insect cells using baculovirus Genebank Number: NM_018323 Applications: PI 4-Kinase assay , western blot. Formulation: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. Storage and stability: Store product at -80ºC. Stable up to one year Product Description: PI4KIIB is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4KIIB is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses. PI4KIIB uses phosphatidylinositol as their primary substrate and has no activity on phosphatidylinositol monophosphates. PI4KIIB is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation. |