The Primary Cell Optimization 384-well NucleofectorTM Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT NucleofectorTM System.
Complete Optimization: Different conditions can easily be tested within one experiment using a 384-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control. Our five Primary Cell NucleofectorTM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our scientific support team.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell NucleofectorTM Solutions and NucleofectorTM Programs. Basic Protocols are available for following cell groups: