The Cell Line Optimization 4D-NucleofectorTM X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-NucleofectorTM X Unit.
With the unique capability of the 4D-NucleofectorTM X Unit to address individual wells of a 16-well NucleocuvetteTM Strip with different programs, cell line optimizations are easily performed within one experiment. Using three 16-well NucleocuvetteTM Strips and our pmaxGFPTM Vector as positive control, our three Cell Line NucleofectorTM Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well NucleocuvetteTM Strip can be used for optional fine tuning or performing the first experiment with the selected conditions.