The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different human stem cell clones, we have developed two NucleofectorTM Kits (1 and 2) and an easy-to-use NucleofectorTM Starter Kit. The Human Stem Cell NucleofectorTM Starter Kit is used to determine which one of our two NucleofectorTM Kits is best suited for transfecting a specific primary human stem cell clone in NucleofectorTM 2b Device. It is suitable for 18 optimization reactions.