In addition to various cell type-specific kits for primary epithelial cells, we also provide a cell group-specific Basic NucleofectorTM Kit. This kit is suited to determine the optimal transfection conditions for any mammalian epithelial cell type that lacks a cell type specific Optimized Protocol for the NucleofectorTM 2b Device (or older generations I or II). In a first experiment a selection of different NucleofectorTM Programs is tested in combination with the Basic NucleofectorTM Solution for epithelial cells.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.