The Nucleofector® 2b System (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector® Kit is the optimal kit for efficient transfection of primary rat neurons.
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.