The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Mouse Embryonic Fibroblast NucleofectorTM Kit 1 is one of our two kits suited for efficient transfectiion of primary mouse embyonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two NucleofectorTM Kits (1 and 2) and an easy-to-use NucleofectorTM Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone.
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.