The Basic Parasite NucleofectorTM Kit 2 is one of our two NucleofectorTM Kits suited for transfecting parasites, e.g. Plasmodium berghei or Trypanosoma brucei, in NucleofectorTM 2b Device. Parasitic protozoa infect vertebrates and invertebrates and some are even parasitic in plants. In humans, they can cause severe diseases, such as Malaria (Plasmodium), Sleeping Sickness (Trypanosoma) or Leishmaniasis (Leishmania). Nucleofection has proven to provide considerably higher transfection efficiencies (e.g., in Plasmodium berghei and Trypanosoma brucei) compared to standard methods, such as electroporation or particle bombardment. Due to significant genotypic and phenotypic diversity between species and life cycles, we have developed two Basic Parasite NucleofectorTM Kits (1 and 2) and an easy-to-use Basic Parasite NucleofectorTM Starter Kit to determine which of the 2 kits is optimal for a specific parasite type.