Biotin-SP-conjugated AffiniPure Goat Anti-Rat IgG, Fcγ Fragment Specific 112-065-008

产品介绍

产品名称：Biotin-SP-conjugated AffiniPure Goat Anti-Rat IgG, Fcγ Fragment Specific

产品货号：112-065-008

产品规格：2.0ml

价格：2280

Target: Rat
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG, Fcγ fragment specific
Conjugate: Biotin-SP (long spacer)
Product Category: Whole IgG Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2338170

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins. No antibody was detected against rat IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.




Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:


1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin
1:500 - 1:5,000 for enzyme immunohisto/cytochemistry
1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.