Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Length 4.5 hours Sample Type & Volume Required Per Well Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL) Sensitivity 0.043 ng/mL Assay Range 0.6 - 20 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine) Specificity Natural and recombinant mouse RBP4 Cross-reactivity < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested. Interference No significant interference observed with available related molecules. |