Purity >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. When recombinant human Slit3 is coated at 250 ng/mL, Recombinant Human ROBO1 Fc Chimera binds with a typical ED50 of 100-600 ng/mL. Source Mouse myeloma cell line, NS0-derived human ROBO1 protein Human ROBO1/DUTT1 (Ser20-Pro858) Accession # NP_598334.1 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus Accession # NP_598334.1 N-terminal Sequence Analysis Ser20 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 119 kDa |