Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level <0.01 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. When Recombinant Human IL 28 R alpha /IFN lambda R1 Fc Chimera (Catalog # 5260-MR) is immobilized at 5.00 μg/mL (100 μL/well), the concentration of Recombinant Human IL-10 R beta Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.300-3.00 μg/mL. Source Mouse myeloma cell line, NS0-derived human IL-10 R beta protein Human IL-10 R beta (Met20-Ser220) Accession # Q08334 DIEGRMD Human IgG1 (Pro100-Lys330) 6-His tag N-terminus C-terminus Accession # Q08334 N-terminal Sequence Analysis Met20 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 51 kDa (monomer) SDS-PAGE 75-85 kDa, reducing conditions |