Purity >90%, by SDS-PAGE with silver staining Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. When recombinant human Slit3 or Recombinant Mouse Slit3 (Catalog # 3629-SL) is coated at 0.25 μg/mL, Recombinant Mouse ROBO2 Fc Chimera binds with an apparent Kd <2 nM. Source Mouse myeloma cell line, NS0-derived mouse ROBO2 protein Mouse ROBO2 (Ser22-Ala863) Accession # NP_780758 IEGRMDP Mouse IgG2A (Glu98-Lys330) N-terminus C-terminus Accession # NP_780758 N-terminal Sequence Analysis Ser22 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 120 kDa SDS-PAGE 100-155 kDa, reducing conditions |