Purity >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834. The ED50 for this effect is 0.4-2 μg/mL. Source Human embryonic kidney cell, HEK293-derived human SR-BI protein Human SR-BI (Pro33-Tyr443) Accession # CAA80277 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus Accession # CAA80277 N-terminal Sequence Analysis Pro33 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 73 kDa SDS-PAGE 84-115 kDa, reducing conditions |