Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain Endotoxin Level <0.1 EU per 1 μg of the protein by the LAL method. Activity Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 3-18 pg/mL. Source E. coli-derived rabbit IL-1 beta/IL-1F2 protein Ala117-Ser268 Accession # NP_001075670 N-terminal Sequence Analysis Ala117 Predicted Molecular Mass 17.5 kDa SDS-PAGE 19 kDa, reducing conditions |