Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 3-15 ng/mL. Source E. coli-derived rabbit IL-4 protein Arg25-Ser147, with an N-terminal Met Accession # NP_001156649 N-terminal Sequence Analysis Met Predicted Molecular Mass 14.2 kDa SDS-PAGE 12.5 kDa, reducing conditions |