Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. When recombinant mouse (rm) IL-10 R alpha is present at 1 µg/mL, it binds rmIL-10 R beta Fc Chimera in the presence of rmIL-10. The concentration of rmIL-10 R beta Fc Chimera that produces 50% of the optimal binding response is approximately 0.25-1.25 μg/mL. Source Mouse myeloma cell line, NS0-derived mouse IL-10 R beta protein Mouse IL-10 R beta (Ile21-Ser220) Accession # Q61190 IEGRMDP Mouse IgG2A (Glu98-Lys330) N-terminus C-terminus Accession # Q61190 N-terminal Sequence Analysis Ile21 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 50.5 kDa (monomer) SDS-PAGE 70-80 kDa, reducing conditions |