产品介绍
| Purity >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level <0.01 EU per 1 μg of the protein by the LAL method. Activity Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 1.5-6.0 µg/mL in the presence of 10 ng/mL Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB). Source Mouse myeloma cell line, NS0-derived human ErbB3/Her3 protein Human ErbB3 (Ser20-Thr643) Accession # P21860 IEGRMD Human IgG1 (Pro100-Lys330) 6-His tag N-terminus C-terminus Accession # P21860 N-terminal Sequence Analysis Ser20 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 96 kDa (monomer) SDS-PAGE 130-140 kDa, reducing conditions |