Purity >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. When Recombinant Mouse Slit3 (Catalog # 9296-SL) is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Mouse ROBO3 Fc Chimera that produces 50% of the optimal binding response is 0.1-0.7 μg/mL Source Mouse myeloma cell line, NS0-derived mouse ROBO3 protein Mouse ROBO3 (Gly54-Ser545) Accession # Q9Z2I4 IEGRMDP Mouse IgG2A (Glu98-Lys330) N-terminus C-terminus Accession # Q9Z2I4 N-terminal Sequence Analysis Gly54 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 81 kDa (monomer) SDS-PAGE 100-110 kDa, reducing conditions |