Purity >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. Biotinylated recombinant mouse Slit-3 immobilized on a streptavidin-coated plate at 1 µg/mL can bind Recombinant Human ROBO2 Fc Chimera with an apparent Kd <10 nM. Source Mouse myeloma cell line, NS0-derived human ROBO2 protein Human ROBO2 (Ser22-Pro863) Accession # NP_001276969 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus Accession # NP_001276969 N-terminal Sequence Analysis Ser22 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 120 kDa (monomer) SDS-PAGE 146-160 kDa, reducing conditions |