Purity >90%, by SDS-PAGE under reducing conditions and visualized by silver stain Endotoxin Level <0.01 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. Biotinylated recombinant mouse Slit-3 captured on a streptavidin-coated plate at 1 µg/mL can bind Recombinant Human ROBO3 Fc Chimera with an apparent Kd <10 nM. Source Mouse myeloma cell line, NS0-derived human ROBO3 protein Human ROBO3 (Leu40-Ser545) Accession # Q96MS0 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus Accession # Q96MS0 N-terminal Sequence Analysis Leu40 Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 82 kDa (monomer) SDS-PAGE 95-120 kDa, reducing conditions |