Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain Endotoxin Level <0.1 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. When recombinant human ROBO4 Fc Chimera is immobilized at 5 μg/mL, biotinylated recombinant rat UNC5H2/Fc Chimera binds with an apparent KD < 20 nM. Source Mouse myeloma cell line, NS0-derived human ROBO4 protein Human ROBO4 (Gln28 - Arg467) Accession # Q8WZ75 IEGRMD Human IgG1 (Pro100 - Lys330) N-terminus C-terminus Accession # Q8WZ75 N-terminal Sequence Analysis No results obtained: Gln28 predicted Structure / Form Disulfide-linked homodimer Predicted Molecular Mass 74 kDa (monomer) SDS-PAGE 90-100 kDa, reducing conditions |