Purity >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method. Activity Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 2‑10 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4 (Catalog # 204-IL). Source Spodoptera frugiperda, Sf 21 (stably transfected)-derived human IL-4R alpha protein Met1-His232 Accession # P24394 N-terminal Sequence Analysis Gly24 Predicted Molecular Mass 24 kDa SDS-PAGE 26-39 kDa, reducing conditions |