Purity >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Activity Measured by its binding ability in a functional ELISA. When Recombinant Rat C-Reactive Protein/CRP is present at 1 μg/mL, the concentration of Recombinant Human Fc gamma RIIA/CD32a (Catalog # 1330-CD) that produces 50% of the optimal binding response is 0.5-3 μg/mL. Source Mouse myeloma cell line, NS0-derived rat C-Reactive Protein/CRP protein His20-Ser230, with a C-terminal 6-His tag Accession # P48199 N-terminal Sequence Analysis His20 Structure / Form Homopentamer Predicted Molecular Mass 24 kDa (monomer) SDS-PAGE 25-38 kDa, reducing conditions |