ab230932 ; Mouse Apo B ELISA Kit ; 1x 384 test

产品介绍

产品名称 小鼠Apo B ELISA试剂盒 参阅全部 Apolipoprotein B 试剂盒 检测方法 Colorimetric 精确度 批次内 样品 n Mean SD CV% Serum 8   2.4% 批次间 样品 n Mean SD CV% Serum 3   3.9% 样品类型 Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma 检测类型 Sandwich (quantitative) 灵敏度 196 pg/ml 范围 1.56 ng/ml - 100 ng/ml 回收率 特定样本回收率 样品类型 平均% 范围 Serum 83 75% - 95% Cell culture media 104 99% - 107% Hep Plasma 98 91% - 107% EDTA Plasma 88 79% - 98% Cit plasma 86 75% - 99% 检测时间 1h 30m 实验步骤 One step assay 种属反应性 与反应: Mouse 产品概述 Mouse Apo B ELISA Kit (ab230932) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apo B protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Apo B with 196 pg/ml sensitivity. SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:         - Single-wash protocol reduces assay time to 90 minutes or less         - High sensitivity, specificity and reproducibility from superior antibodies         - Fully validated in biological samples         - 96-wells plate breakable into 12 x 8 wells strips A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits. ASSAY SPECIFICITY This kit recognizes both native and recombinant mouse ApoB protein in serum, plasma, and cell culture supernatant samples only. SPECIES REACTIVITY This kit recognizes native and recombinant mouse ApoB protein. Other species reactivity was determined by measuring human serum samples, interpolating the protein concentrations from the standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in serum assayed at the same dilution. Reactivity < 3% was determined for Human 经测试应用 适用于: Sandwich ELISAmore details 平台 Pre-coated microplate (12 x 8 well strips)