产品名称 人PTH ELISA试剂盒 参阅全部 Parathyroid Hormone 试剂盒 检测方法 Colorimetric 精确度 批次内 样品 n Mean SD CV% Spiked serum 8 1.5% 批次间 样品 n Mean SD CV% Spiked serum 3 3.8% 样品类型 Serum, Hep Plasma, EDTA Plasma, Cit plasma 检测类型 Sandwich (quantitative) 灵敏度 0.761 pg/ml 范围 4.69 pg/ml - 300 pg/ml 回收率 特定样本回收率 样品类型 平均% 范围 Serum 88 88% - 89% Cell culture media 105 104% - 106% Hep Plasma 83 81% - 84% EDTA Plasma 90 88% - 92% Cit plasma 82 81% - 82% 检测时间 1h 30m 实验步骤 One step assay 种属反应性 与反应: Human 产品概述 Human PTH ELISA Kit (ab230931) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PTH protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PTH with 0.761 pg/ml sensitivity. SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits: - Single-wash protocol reduces assay time to 90 minutes or less - High sensitivity, specificity and reproducibility from superior antibodies - Fully validated in biological samples - 96-wells plate breakable into 12 x 8 wells strips A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits. 经测试应用 适用于: Sandwich ELISAmore details 平台 Pre-coated microplate (12 x 8 well strips) |