2-Benzoxazolinone is a metabolite produced by rye (Secale cereale) that inhibits germination and growth and causing oxidative damage. 2-Benzoxazolinone has diverse pharmacological activities including antinociception, anti-inflammatory, antileishmanial, antioxidant, anti-HIV, anticancer, antidepressant, and neuroprotective effects. 2-Benzoxazolinone is a sought-after structure for the design of bioactive molecules. 2-Benzoxazolinone is soluble in methanol and DMSO.