>400 µg total serum protein mass (> 85% Albumin, >85% IgG depleted) from 25 µl serum prep
Affinity-type equivalence, virtually no cross-reactivity with other proteins
Disposable, no column regeneration or cross-contamination
Combines unique bead technology, not based on Blue-dye affinity, with optimized Protein A
Mild conditions maintains structural integrity and simple transfer to secondary analysis
Suitable for immunoassay, Western blot, 1 & 2D Electrophoresis, enzyme assay, LC-MS
Tested species include human, sheep, bovine, rabbit, mouse, rat
Validated in the automation compatible high-throughput DPX Technologies XTR tip format
Poly-electrolytes are polymers with repeating units of stationary charges. AlbuSorb™ comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. AlbuSorb™ combines with an optimized immobilized Protein A to create AlbuSorb™ PLUS .
Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination. AlbuSorb™ PLUS is supplied as a powder. Simply weigh, centrifuge and/or filter, and recover the {albumin + Immunoglobulin} - depleted serum in the supernatant.