1ml (10x) Protein Sample Reducing Reagent (1 M DTT) For 20 µl sample loading volume: add 4 µl 5x SDS protien sample loading buffer, 2 µl 10x Protein sample reducing reagent, appropriate amount of protein (20-50 µg for cell lysate; 20-50 ng for purified protein), ddH2O to final volume. > |