DG-013-0160 ; 7-deaza-dGTP

产品介绍

Application G residues can form secondary structures which are not fully denatured during electrophoresis. This can lead to sequence ambiguities. 7-Deaza-G doesn't form these secondary structures, so avoids sequence ambiguities. Description 7-deaza-2′-deoxyguanosine-5′-triphosphate 7-deaza-dGTP may be effectively incorporated into the DNA by DNA polymerases and/or terminal deoxynucleotidyl transferase. 7-deaza-dG-containing DNA duplexes demonstrate increased stability, 7-deaza-dG-containing DNA penetrates cell membrane more effectively due to the increased lypophility. 7-deaza dGTP may be used in sequencing for the resolution of the compressions in G-C-rich regions. Amplification of some DNA regions is often hindered due to the high G-C content. Replacement of dGTP to 7-deaza dGTP in the master mix may solve the problem. Concentration 1 µmol (100 µl of 10 mM solution) Formula C11H17N4O13P3,  Molecular Weight (506.2) Purity 96-99% (by HPLC) Storage Store at -20 oC