The 100 bp DNA marker is suitable for sizing linear double-stranded DNA fragments from 100 to 1000 bp. The 10 bands of the ladder contain fragments with the following sizes: 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 bp The recommended amount of size marker to load on an agarose gel are 0.5-1 µ(5-10 µl). To demonstrate the mobility of the DNA fragments, 1 µg of marker was loaded onto a 1% agarose gel. CONCENTRATION 1 µg/10 µl SIZE 50 µg in 500 µl (100-200 applications) loading buffer (0.25% bromphenoblue, 0.25% xylene cyanol FF, 40% sucrose in water) STORAGE Room temperature |