Pradofloxacin is a third-generation enhanced spectrum antibiotic belonging to the Fluoroquinolone class, specifically 8-cyano-fluoroquinolone. It was developed by Bayer Healthcare in 1994 to improve upon the efficacy against Gram-positive and anaerobic pathogens compared to previous generation fluoroquinolones while delivering the same Gram-negative activity. The compound is used for in vitro veterinary research applications.