Name Elite™ cAMP ELISA Kit (Fluorescence) Description Elite™ cAMP ELISA Kit provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared with other cAMP ELISA kits, our kit eliminates the tedious acetylation step. The kit uses Elite™ Red as a fluorimetric substrate to quantify the HRP activity. The high sensitivity (for as low as 0.1 nM) makes it superior to any other known colorimetric-based or fluorescence-based kits in the market. Application The assay can be conveniently performed in a 96-well microtiter-plate format and can be easily adapted to automation. The fluorescent product formed is proportional to the activity of the HRP-cAMP conjugate. Sensitivity 0.1 pmol/mL to 10 pmol/mL; EC50 = 1.6 pmol/mL Size 1 plate (96-well) Detection Fluorescence microplate reader |